| Chapter title |
Chromosome Architecture
|
|---|---|
| Chapter number | 18 |
| Book title |
Chromosome Architecture
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3631-1_18 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3629-8, 978-1-4939-3631-1
|
| Authors |
Récamier, Vincent, Vincent Récamier |
| Editors |
Mark C. Leake |
| Abstract |
Chromosome architecture needs to be investigated in relation with the chemical function of DNA. The kinetics of gene expression, DNA replication, and repair are driven by the mechanisms by which a functional nuclear protein finds its substrate in the nucleus. Single-particle tracking (SPT) is a method to quantify fluorescent molecules dynamics from the tracks of the single molecules recorded by high-resolution microscopes. SPT offers direct observation of the movement and single-molecule resolution. Usually SPT is performed on membranes because of higher contrast. Here, we introduce a novel method to record the trajectories of weakly fluorescent molecules in the nucleus of living cells. I-SPT uses some specific detection and analysis tools to enable the computation of reliable statistics on nuclear particle movement. |
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