Chapter title |
Chromosome Architecture
|
---|---|
Chapter number | 2 |
Book title |
Chromosome Architecture
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3631-1_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3629-8, 978-1-4939-3631-1
|
Authors |
Wollman, Adam J M, Leake, Mark C, Adam J. M. Wollman, Mark C. Leake |
Editors |
Mark C. Leake |
Abstract |
Single-molecule narrow-field microscopy is a versatile tool to investigate a diverse range of protein dynamics in live cells and has been extensively used in bacteria. Here, we describe how these methods can be extended to larger eukaryotic, yeast cells, which contain subcellular compartments. We describe how to obtain single-molecule microscopy data but also how to analyze these data to track and obtain the stoichiometry of molecular complexes diffusing in the cell. We chose glucose mediated signal transduction of live yeast cells as the system to demonstrate these single-molecule techniques as transcriptional regulation is fundamentally a single-molecule problem-a single repressor protein binding a single binding site in the genome can dramatically alter behavior at the whole cell and population level. |
X Demographics
Geographical breakdown
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Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 17 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 5 | 29% |
Professor | 2 | 12% |
Student > Master | 2 | 12% |
Researcher | 2 | 12% |
Student > Postgraduate | 1 | 6% |
Other | 0 | 0% |
Unknown | 5 | 29% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 4 | 24% |
Physics and Astronomy | 4 | 24% |
Biochemistry, Genetics and Molecular Biology | 1 | 6% |
Neuroscience | 1 | 6% |
Chemistry | 1 | 6% |
Other | 1 | 6% |
Unknown | 5 | 29% |