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Chromosome Architecture

Overview of attention for book
Attention for Chapter 12: Escherichia coli Chromosome Copy Number Measurement Using Flow Cytometry Analysis.
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Chapter title
Escherichia coli Chromosome Copy Number Measurement Using Flow Cytometry Analysis.
Chapter number 12
Book title
Chromosome Architecture
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3631-1_12
Pubmed ID
Book ISBNs
978-1-4939-3629-8, 978-1-4939-3631-1
Authors

Michelle Hawkins, John Atkinson, Peter McGlynn

Editors

Mark C. Leake

Abstract

Flow cytometry is a high-throughput technique that analyzes individual particles as they pass through a laser beam. These particles can be individual cells and by detecting cell-scattered light their number and relative size can be measured as they pass through the beam. Labeling of molecules, usually via a fluorescent reporter, allows the amount of these molecules per cell to be quantified. DNA content can be estimated using this approach and here we describe how flow cytometry can be used to assess the DNA content of Escherichia coli cells.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 12 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 25%
Researcher 2 17%
Other 1 8%
Student > Doctoral Student 1 8%
Lecturer 1 8%
Other 2 17%
Unknown 2 17%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 7 58%
Agricultural and Biological Sciences 2 17%
Neuroscience 1 8%
Unknown 2 17%