Chapter title |
Modification of Expanded NK Cells with Chimeric Antigen Receptor mRNA for Adoptive Cellular Therapy.
|
---|---|
Chapter number | 18 |
Book title |
Natural Killer Cells
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Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3684-7_18 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3682-3, 978-1-4939-3684-7
|
Authors |
Yaya Chu, Allyson Flower, Mitchell S. Cairo M.D., Mitchell S. Cairo |
Editors |
Srinivas S. Somanchi |
Abstract |
NK cells are bone marrow-derived cytotoxic lymphocytes that play a major role in the rejection of tumors and cells infected by viruses. The regulation of NK activation vs inhibition is regulated by the expression of a variety of NK receptors (NKRs) and specific NKRs' ligands expressed on their targets. However, factors limiting NK therapy include small numbers of active NK cells in unexpanded peripheral blood and lack of specific tumor targeting. Chimeric antigen receptors (CAR) usually include a single-chain Fv variable fragment from a monoclonal antibody, a transmembrane hinge region, and a signaling domain such as CD28, CD3-zeta, 4-1BB (CD137), or 2B4 (CD244) endodimers. Redirecting NK cells with a CAR will circumvent the limitations of the lack of NK targeting specificity. This chapter focuses on the methods to expand human NK cells from peripheral blood by co-culturing with feeder cells and to modify the expanded NK cells efficiently with the in vitro transcribed CAR mRNA by electroporation and to test the functionality of the CAR-modified expanded NK cells for use in adoptive cellular immunotherapy. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 16 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 4 | 25% |
Student > Doctoral Student | 2 | 13% |
Student > Master | 2 | 13% |
Professor | 1 | 6% |
Student > Bachelor | 1 | 6% |
Other | 1 | 6% |
Unknown | 5 | 31% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 25% |
Medicine and Dentistry | 2 | 13% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 13% |
Veterinary Science and Veterinary Medicine | 1 | 6% |
Agricultural and Biological Sciences | 1 | 6% |
Other | 1 | 6% |
Unknown | 5 | 31% |