Chapter title |
Natural Killer Cells
|
---|---|
Chapter number | 7 |
Book title |
Natural Killer Cells
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3684-7_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3682-3, 978-1-4939-3684-7
|
Authors |
Nandagopal, Saravanan, Lin, Francis, Kung, Sam K P, Saravanan Nandagopal, Francis Lin, Sam K. P. Kung |
Editors |
Srinivas S. Somanchi |
Abstract |
Effector functions and cellular properties of natural killer (NK) cells are regulated by cellular and extracellular factors shaped by the microenvironments. NK cells express specific chemokine and non-chemokine receptors to aid preferential migrations or localizations in tissues. Good understanding of how NK-cell migratory properties are regulated in physiological and pathological microenvironments will provide further insights into the development of NK cell-based therapeutic approaches. In contrast to the commonly used conventional in vitro migration assays such as Trans-well assays that measure movements of a population of the migratory cells, microfluidic-based devices support live-cell imaging of cell migrations under a well-defined chemical gradient(s) at microscale. Subsequent analyses at single-cell level provide quantitative measurements of cell-migration parameters such as speed and Chemotactic Index, and permit distinguishing chemotaxis, chemokinesis, and chemo-repulsion. Our recent work established the use of a Y-shaped microfluidic device to study NK cell migrations in vitro. In this chapter, we described the detailed method of acquiring and analyzing NK cell migration in the microfluidic devices. |
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