Chapter title |
Natural Killer Cells
|
---|---|
Chapter number | 10 |
Book title |
Natural Killer Cells
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3684-7_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3682-3, 978-1-4939-3684-7
|
Authors |
Theorell, Jakob, Bryceson, Yenan T, Jakob Theorell, Yenan T. Bryceson |
Editors |
Srinivas S. Somanchi |
Abstract |
In signaling cascades downstream of NK cell activating receptor engagement, Ca(2+) ions are pivotal second messengers for NK cell cytotoxicity as well as cytokine production. Upon cellular activation, intracellular mobilization of Ca(2+) ions initially involves depletion of endoplasmic reticulum stores, leading to subsequent Ca(2+) influx through specific plasma membrane Ca(2+) release activated Ca(2+) channels. Multiple probes and assays for detecting intracellular Ca(2+) concentrations have been developed. With the advance of multiparameter flow cytometry instrumentation, a thorough analysis of signaling in specific NK cell subsets is possible. Here, a flow cytometric method for dynamic measurements of intracellular Ca(2+) concentrations in human NK cells subsets is detailed and discussed. This assay can be further adapted for specific scientific and diagnostic questions, with implications for various immunopathological conditions. |
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Mendeley readers
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Unknown | 4 | 40% |