| Chapter title |
Natural Killer Cells
|
|---|---|
| Chapter number | 20 |
| Book title |
Natural Killer Cells
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3684-7_20 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3682-3, 978-1-4939-3684-7
|
| Authors |
Lowe, Emily, Truscott, Laurel C, De Oliveira, Satiro N, Emily Lowe Ph.D., Laurel C. Truscott M.D., Satiro N. De Oliveira M.D., Emily Lowe, Laurel C. Truscott, Satiro N. De Oliveira |
| Editors |
Srinivas S. Somanchi |
| Abstract |
NK cells represent a very promising source for adoptive cellular approaches for cancer immunotherapy, and extensive research has been conducted, including clinical trials. Gene modification of NK cells can direct their specificity and enhance their function, but the efficiency of gene transfer techniques is very limited. Here we describe two protocols designed to generate mature human NK cells from gene-modified hematopoietic stem cells. These protocols use chimeric antigen receptor as the transgene, but could potentially be modified for the expression any particular transgene in human NK cells. |
X Demographics
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| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 31 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 8 | 26% |
| Student > Master | 6 | 19% |
| Student > Bachelor | 4 | 13% |
| Other | 3 | 10% |
| Student > Doctoral Student | 2 | 6% |
| Other | 3 | 10% |
| Unknown | 5 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 9 | 29% |
| Immunology and Microbiology | 4 | 13% |
| Agricultural and Biological Sciences | 3 | 10% |
| Engineering | 3 | 10% |
| Biochemistry, Genetics and Molecular Biology | 1 | 3% |
| Other | 5 | 16% |
| Unknown | 6 | 19% |