Chapter title |
Natural Killer Cells
|
---|---|
Chapter number | 12 |
Book title |
Natural Killer Cells
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3684-7_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3682-3, 978-1-4939-3684-7
|
Authors |
Mace, Emily M, Orange, Jordan S, Emily M. Mace PhD, Jordan S. Orange M.D., Ph.D., Emily M. Mace, Jordan S. Orange |
Editors |
Srinivas S. Somanchi |
Abstract |
Recent advances in imaging technology have enabled significant advances in the study of NK cell cytotoxic effector function through quantitative analysis of the NK cell immunological synapse. This can include the use of high- and super-resolution microscopy to quantify dynamics of cytoskeletal elements and the role they play in the regulation and execution of NK cell directed secretion. Here we describe a protocol for the recapitulation of the NK cell lytic synapse on glass, the acquisition of microscopy images, and suggested approaches for the processing and analysis of microscopy data. |
X Demographics
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France | 1 | 20% |
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Demographic breakdown
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Scientists | 1 | 20% |
Mendeley readers
Geographical breakdown
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Unknown | 12 | 100% |
Demographic breakdown
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Unspecified | 1 | 8% |
Student > Doctoral Student | 1 | 8% |
Professor | 1 | 8% |
Student > Master | 1 | 8% |
Other | 2 | 17% |
Unknown | 2 | 17% |
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Unspecified | 1 | 8% |
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Immunology and Microbiology | 1 | 8% |
Other | 2 | 17% |
Unknown | 3 | 25% |