Chapter title |
Isolation and Selection of New Astaxanthin-Producing Strains of Phaffia rhodozyma
|
---|---|
Chapter number | 18 |
Book title |
Microbial Carotenoids
|
Published in |
Methods in molecular biology, August 2018
|
DOI | 10.1007/978-1-4939-8742-9_18 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8741-2, 978-1-4939-8742-9
|
Authors |
Diego Libkind, Martín Moliné, Fernando Colabella, Libkind, Diego, Moliné, Martín, Colabella, Fernando |
Abstract |
Astaxanthin is a xanthophyll pigment of high economic value for its use as a feeding component in aquaculture. Phaffia rhodozyma (Xanthophyllomyces dendrorhous) is a basidiomycetous fungi able to synthesize astaxanthin as its major carotenoid, the only known yeast species bearing the capability to produce this type of carotenoid and the only tremellomycetes with biotechnological application. Recently, the habitat and intraspecific variability of this species have been found to be wider than previously expected, encouraging the search for new wild strains with potential biotechnological applications. Here we describe effective procedures for isolation of P. rhodozyma from environmental samples, accurate identification of the strains, analysis of their astaxanthin content, and proper conservation of the isolates. |
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