| Chapter title |
Synthesis of Carotenoids of Industrial Interest in the Photosynthetic Bacterium Rhodopseudomonas palustris : Bioengineering and Growth Conditions
|
|---|---|
| Chapter number | 12 |
| Book title |
Microbial Carotenoids
|
| Published in |
Methods in molecular biology, August 2018
|
| DOI | 10.1007/978-1-4939-8742-9_12 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8741-2, 978-1-4939-8742-9
|
| Authors |
Eric Giraud, Laure Hannibal, Clémence Chaintreuil, Joël Fardoux, André Verméglio, Giraud, Eric, Hannibal, Laure, Chaintreuil, Clémence, Fardoux, Joël, Verméglio, André |
| Abstract |
Rhodopseudomonas palustris is a purple photosynthetic bacterium that accumulates in the inner membrane the photosynthetic pigment spirilloxanthin, formed from lycopene. Here, we describe the procedures used to successfully engineer Rps. palustris strains to reroute the production of lycopene toward the synthesis of ß-carotene or canthaxanthin. The crtCD genes specifically involved in spirilloxanthin were replaced by crtY and crtW genes from Bradyrhizobium ORS278 to synthesize ß-carotene and (or) canthaxanthin, two pigments of industrial interest. Since the synthesis of canthaxanthin depends on the presence of oxygen, the procedure to optimize their production is also proposed. By modulating the light and oxygen during the growth process, a single species of photosynthetic bacteria, with an efficient growth rate, produces various carotenoids of economical interest. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 15 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 20% |
| Student > Master | 3 | 20% |
| Student > Ph. D. Student | 2 | 13% |
| Student > Doctoral Student | 2 | 13% |
| Student > Bachelor | 1 | 7% |
| Other | 0 | 0% |
| Unknown | 4 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 6 | 40% |
| Agricultural and Biological Sciences | 3 | 20% |
| Chemical Engineering | 2 | 13% |
| Unknown | 4 | 27% |