Chapter title |
Minocycline Attenuates Iron-Induced Brain Injury
|
---|---|
Chapter number | 62 |
Book title |
Brain Edema XVI
|
Published in |
Acta neurochirurgica Supplement, January 2016
|
DOI | 10.1007/978-3-319-18497-5_62 |
Pubmed ID | |
Book ISBNs |
978-3-31-918496-8, 978-3-31-918497-5
|
Authors |
Zhao, Fan, Xi, Guohua, Liu, Wenqaun, Keep, Richard F, Hua, Ya, Fan Zhao, Guohua Xi, Wenqaun Liu, Richard F. Keep, Ya Hua |
Abstract |
Iron plays an important role in brain injury after intracerebral hemorrhage (ICH). Our previous study found minocycline reduces iron overload after ICH. The present study examined the effects of minocycline on the subacute brain injury induced by iron. Rats had an intracaudate injection of 50 μl of saline, iron, or iron + minocycline. All the animals were euthanized at day 3. Rat brains were used for immunohistochemistry (n = 5-6 per each group) and Western blotting assay (n = 4). Brain swelling, blood-brain barrier (BBB) disruption, and iron-handling proteins were measured. We found that intracerebral injection of iron resulted in brain swelling, BBB disruption, and brain iron-handling protein upregulation (p < 0.05). The co-injection of minocycline with iron significantly reduced iron-induced brain swelling (n = 5, p < 0.01). Albumin, a marker of BBB disruption, was measured by Western blot analysis. Minocycline significantly decreased albumin protein levels in the ipsilateral basal ganglia (p < 0.01). Iron-handling protein levels in the brain, including ceruloplasmin and transferrin, were reduced in the minocycline co-injected animals. In conclusion, the present study suggests that minocycline attenuates brain swelling and BBB disruption via an iron-chelation mechanism. |
X Demographics
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Unknown | 2 | 100% |
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Members of the public | 2 | 100% |
Mendeley readers
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Unknown | 13 | 100% |
Demographic breakdown
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Other | 2 | 15% |
Professor > Associate Professor | 2 | 15% |
Student > Master | 1 | 8% |
Student > Bachelor | 1 | 8% |
Student > Doctoral Student | 1 | 8% |
Other | 2 | 15% |
Unknown | 4 | 31% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 2 | 15% |
Medicine and Dentistry | 2 | 15% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 8% |
Neuroscience | 1 | 8% |
Chemistry | 1 | 8% |
Other | 2 | 15% |
Unknown | 4 | 31% |