Chapter title |
Cannabinoid Receptor Type 2 Agonist Attenuates Acute Neurogenic Pulmonary Edema by Preventing Neutrophil Migration after Subarachnoid Hemorrhage in Rats.
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Chapter number | 24 |
Book title |
Brain Edema XVI
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Published in |
Acta neurochirurgica Supplement, January 2016
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DOI | 10.1007/978-3-319-18497-5_24 |
Pubmed ID | |
Book ISBNs |
978-3-31-918496-8, 978-3-31-918497-5
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Authors |
Fujii, Mutsumi, Sherchan, Prativa, Soejima, Yoshiteru, Doycheva, Desislava, Zhao, Diana, Zhang, John H, Mutsumi Fujii, Prativa Sherchan, Yoshiteru Soejima, Desislava Doycheva, Diana Zhao, John H. Zhang |
Abstract |
We evaluated whether JWH133, a selective cannabinoid type 2 receptor (CB2R) agonist, prevented neurogenic pulmonary edema (NPE) after subarachnoid hemorrhage (SAH) by attenuating inflammation. Adult male rats were assigned to six groups: sham-operated, SAH with vehicle, SAH with JWH133 (0.3, 1.0, or 3.0 mg/kg) treatment 1 h after surgery, and SAH with JWH133 (1.0 mg/kg) at 1 h with a selective CB2R antagonist, SR144528 (3.0 mg/kg). The perforation model of SAH was performed and pulmonary wet-to-dry weight ratio was evaluated 24 and 72 h after surgery. Western blot analyses and immunohistochemistry were evaluated 24 h after surgery. JWH133 (1.0 mg/kg) significantly and most strongly improved lung edema 24 h after SAH. SR144528 administration significantly reversed the effects of JWH133 (1.0 mg/kg). SAH-induced increasing levels of myeloperoxidase (MPO) and decreasing levels of a tight junction (TJ) protein, junctional adhesion molecule (JAM)-A, were ameliorated by JWH133 (1.0 mg/kg) administration 24 h after SAH. Immunohistochemical assessment also confirmed substantial leukocyte infiltration in the outside of vessels in SAH, which were attenuated by JWH133 (1.0 mg/kg) injection. CB2R agonist ameliorated lung permeability by inhibiting leukocyte trafficking and protecting tight junction proteins in the lung of NPE after SAH. |
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Mendeley readers
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Unknown | 12 | 100% |
Demographic breakdown
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Researcher | 2 | 17% |
Student > Bachelor | 1 | 8% |
Other | 1 | 8% |
Student > Doctoral Student | 1 | 8% |
Unknown | 7 | 58% |
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Medicine and Dentistry | 2 | 17% |
Biochemistry, Genetics and Molecular Biology | 1 | 8% |
Neuroscience | 1 | 8% |
Agricultural and Biological Sciences | 1 | 8% |
Unknown | 7 | 58% |