Chapter title |
Role of Protease-Activated Receptor-1 in Glioma Growth.
|
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Chapter number | 61 |
Book title |
Brain Edema XVI
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Published in |
Acta neurochirurgica Supplement, January 2016
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DOI | 10.1007/978-3-319-18497-5_61 |
Pubmed ID | |
Book ISBNs |
978-3-31-918496-8, 978-3-31-918497-5
|
Authors |
Xie, Qing, Bao, Xuhui, Chen, Zhan Hong, Xu, Ying, Keep, Richard F, Muraszko, Karin M, Xi, Guohua, Hua, Ya, Qing Xie, Xuhui Bao, Zhan Hong Chen, Ying Xu, Richard F. Keep, Karin M. Muraszko, Guohua Xi, Ya Hua |
Abstract |
Activation of a thrombin receptor, protease-activated receptor-1 (PAR-1), induces angiogenesis, cell proliferation, and invasion in tumors. The present study examined the effect of host PAR-1 gene deletion on glioma growth in a mouse model. F98 glioma cells were implanted into the right caudate of either wild type (WT) or PAR-1 knockout (KO) mice. Mice underwent magnetic resonance imaging (MRI) and the brains were used for measurements of brain water content and tumor mass. Levels of hypoxia-inducible factor-1 alpha (HIF-1α) and vascular endothelial growth factor (VEGF) were also measured by ELISA (enzyme-linked immunosorbent assay). We found brain water content in the ipsilateral hemisphere and the tumor mass were significantly lower in PAR-1 KO than WT mice at day 12 after implantation of F98 cells (p < 0.05). HIF-1α protein levels in the ipsilateral hemisphere were higher in the WT mice (373 ± 25 pg/g brain tissue vs 333 ± 35 pg/g brain tissue in PAR-1 KO mice, p < 0.05) 7 days after F98 cell implantation. VEGF protein levels were also higher in the ipsilateral hemisphere of WT mice (219 ± 21 vs 166 ± 22 pg/g brain tissue in PAR-1 KO mice, p < 0.01). In conclusion, PAR-1 has a role in glioma growth and could be a new therapeutic target for gliomas. |
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Unknown | 1 | 100% |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
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Unknown | 17 | 100% |
Demographic breakdown
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Student > Ph. D. Student | 2 | 12% |
Other | 1 | 6% |
Student > Bachelor | 1 | 6% |
Student > Master | 1 | 6% |
Researcher | 1 | 6% |
Other | 1 | 6% |
Unknown | 10 | 59% |
Readers by discipline | Count | As % |
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Neuroscience | 2 | 12% |
Biochemistry, Genetics and Molecular Biology | 1 | 6% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 6% |
Medicine and Dentistry | 1 | 6% |
Agricultural and Biological Sciences | 1 | 6% |
Other | 0 | 0% |
Unknown | 11 | 65% |