Chapter title |
Methods to Study the Role of Cdc42, Rac1, and Rac2 in B-Cell Cytoskeletal Responses
|
---|---|
Chapter number | 16 |
Book title |
Rho GTPases
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8612-5_16 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8611-8, 978-1-4939-8612-5
|
Authors |
Natalija Gerasimcik, Lisa S. Westerberg, Eva Severinson, Gerasimcik, Natalija, Westerberg, Lisa S., Severinson, Eva |
Abstract |
B-cell migration and adhesion are critical to form a germinal center response, the site for B-cell production of high-affinity antibodies. Here, we describe two assays that can be used to examine B-cell cytoskeletal responses needed during the germinal center response: B-cell spreading and homotypic adhesion. Spreading of B cells is dependent on Cdc42, while Rac1 and Rac2 are necessary for homotypic adhesion. These in vitro assays can be used to examine functional responses of B cells mediated by the cell cytoskeleton, for example when comparing B cells from different gene knockout animals. |
X Demographics
Geographical breakdown
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 9 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 2 | 22% |
Student > Bachelor | 1 | 11% |
Researcher | 1 | 11% |
Student > Master | 1 | 11% |
Unknown | 4 | 44% |
Readers by discipline | Count | As % |
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Immunology and Microbiology | 2 | 22% |
Biochemistry, Genetics and Molecular Biology | 1 | 11% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 11% |
Agricultural and Biological Sciences | 1 | 11% |
Medicine and Dentistry | 1 | 11% |
Other | 0 | 0% |
Unknown | 3 | 33% |