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Mendeley readers
Chapter title |
High-Throughput Assay for RhoGEFs Based on the Transcreener® GDP Assay
|
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Chapter number | 12 |
Book title |
Rho GTPases
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Published in |
Methods in molecular biology, January 2018
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DOI | 10.1007/978-1-4939-8612-5_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8611-8, 978-1-4939-8612-5
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Authors |
Robert G. Lowery, Meera Kumar, Lowery, Robert G., Kumar, Meera |
Abstract |
We describe a high-throughput screening (HTS)-compatible method for detecting GTPase exchange factor (GEF) activity based on stimulation of GDP formation by Rho GTPases. The method is based on the fact that GDP dissociation is the rate-limiting step in the Rho GTPase catalytic cycle, so by accelerating its release a GEF causes an increase in the steady-state rate of GDP formation. The Transcreener® GDP GTPase Assay, a fluorescence polarization immunoassay (FPIA), is used to detect GDP formation in a homogeneous format. |
Mendeley readers
The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 2 | 40% |
Student > Bachelor | 1 | 20% |
Student > Ph. D. Student | 1 | 20% |
Unknown | 1 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 2 | 40% |
Biochemistry, Genetics and Molecular Biology | 1 | 20% |
Chemistry | 1 | 20% |
Unknown | 1 | 20% |