Chapter title |
Characterization of Genetically Encoded FRET Biosensors for Rho-Family GTPases
|
---|---|
Chapter number | 7 |
Book title |
Rho GTPases
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8612-5_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8611-8, 978-1-4939-8612-5
|
Authors |
Sara K. Donnelly, Veronika Miskolci, Alice M. Garrastegui, Dianne Cox, Louis Hodgson, Donnelly, Sara K., Miskolci, Veronika, Garrastegui, Alice M., Cox, Dianne, Hodgson, Louis |
Abstract |
Genetically encoded FRET-based biosensors are increasingly popular and useful tools for examining signaling pathways with high spatial and temporal resolution in living cells. Here, we show basic techniques used to characterize and to validate single-chain, genetically encoded Förster resonance energy transfer (FRET) biosensors of the Rho GTPase-family proteins. Methods described here are generally applicable to other genetically encoded FRET-based biosensors by modifying the tested conditions to include additional/different regulators and inhibitors, as appropriate for the specific protein of interest. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 14 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 3 | 21% |
Student > Master | 2 | 14% |
Student > Bachelor | 2 | 14% |
Professor | 1 | 7% |
Unknown | 6 | 43% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 3 | 21% |
Agricultural and Biological Sciences | 2 | 14% |
Medicine and Dentistry | 2 | 14% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 7% |
Neuroscience | 1 | 7% |
Other | 0 | 0% |
Unknown | 5 | 36% |