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Mendeley readers
Chapter title |
Assessment of S-Glutathionylated Rac1 in Cells Using Biotin-Labeled Glutathione
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Chapter number | 11 |
Book title |
Rho GTPases
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Published in |
Methods in molecular biology, January 2018
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DOI | 10.1007/978-1-4939-8612-5_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8611-8, 978-1-4939-8612-5
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Authors |
Hannah Edenbaum, Jingyan Han, Edenbaum, Hannah, Han, Jingyan |
Abstract |
Rac1 is a member of the family of small Rho GTPases that are molecular switches governing a variety of fundamental cellular processes, such as cell growth and motility. Its subcellular location and activity are regulated by several posttranslational modifications. S-glutathionylation, the adduction of glutathione to cysteine residues in Rac1, is a redox-dependent thiol modification and is generally associated with oxidative/nitrosative stress, representing a novel mechanism of GTPase regulation. Here, we describe the use of biotin-labeled glutathione to monitor intracellular glutathionylated Rac1 in response to exogenous stimuli. |
Mendeley readers
The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 2 | 50% |
Professor | 1 | 25% |
Unknown | 1 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 1 | 25% |
Medicine and Dentistry | 1 | 25% |
Unknown | 2 | 50% |