Chapter title |
Assessing the Roles of Rho GTPases in Cell DNA Repair by the Nucleotide Excision Repair Pathway
|
---|---|
Chapter number | 22 |
Book title |
Rho GTPases
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8612-5_22 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8611-8, 978-1-4939-8612-5
|
Authors |
Lilian C. Russo, Pault Y. Minaya, Luiz E. Silva, Fábio L. Forti, Russo, Lilian C., Minaya, Pault Y., Silva, Luiz E., Forti, Fábio L. |
Abstract |
Ultraviolet light crossing the ozone layer in the atmospheric barrier affects all forms of living beings on earth. In eukaryotic cells, the nucleotide excision repair (NER) pathway protects the DNA by removing cyclobutane pyrimidine dimers (CPDs) and 6-4-photoproduct (6-4-PP) lesions caused by ultraviolet (UV) light, allowing cells to proliferate. On the other hand, adhesion and invasion processes, primarily regulated by the typical Rho GTPases Rho, Rac, and Cdc42, are also affected by UV radiation effects. Studies focused on determining whether or not these GTPases might affect the NER pathway in different cell models are enlightening and should start with classical experimental methodologies. In this chapter we describe two methods (host cell reactivation assay, or HCR, and slot-blots for CPDs and 6-4-PPs) to assess the direct or indirect involvement of these three GTPases on the NER pathway. |
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Geographical breakdown
Country | Count | As % |
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Unknown | 10 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 2 | 20% |
Student > Doctoral Student | 1 | 10% |
Student > Ph. D. Student | 1 | 10% |
Student > Master | 1 | 10% |
Student > Postgraduate | 1 | 10% |
Other | 0 | 0% |
Unknown | 4 | 40% |
Readers by discipline | Count | As % |
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Computer Science | 1 | 10% |
Neuroscience | 1 | 10% |
Chemistry | 1 | 10% |
Other | 0 | 0% |
Unknown | 2 | 20% |