Chapter title |
Analyzing Rho GTPase-Dependent Processes During Cell Polarization in Brown Algae
|
---|---|
Chapter number | 27 |
Book title |
Rho GTPases
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8612-5_27 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8611-8, 978-1-4939-8612-5
|
Authors |
Whitney Hable, Hable, Whitney |
Abstract |
Zygotes of the fucoid brown algae are useful models for investigating the molecular and cellular mechanisms of cell polarization. These organisms are abundant in the marine intertidal zone, where they grow firmly anchored to rocks. In response to environmental cues like sunlight, zygotes generate asymmetries within the cell that ultimately establish an axis of growth. The transduction of these cues relies on Rac1-mediated signaling that remodels the actin cytoskeleton, alters patterns of endocytosis and secretion, and ultimately prepares the zygote for localized (tip) growth. This chapter presents protocols for obtaining synchronous populations of zygotes, and for detecting changes in filamentous actin arrays, endomembrane patterns, and secretion patterns that occur during light-induced polarization. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 1 | 25% |
Researcher | 1 | 25% |
Student > Master | 1 | 25% |
Unknown | 1 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Arts and Humanities | 1 | 25% |
Agricultural and Biological Sciences | 1 | 25% |
Medicine and Dentistry | 1 | 25% |
Unknown | 1 | 25% |