Chapter title |
Isolation of Rat and Mouse Alveolar Type II Epithelial Cells
|
---|---|
Chapter number | 6 |
Book title |
Lung Innate Immunity and Inflammation
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8570-8_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8569-2, 978-1-4939-8570-8
|
Authors |
Nicole L. Jansing, Jazalle McClendon, Hidenori Kage, Mitsuhiro Sunohara, Juan R. Alvarez, Zea Borok, Rachel L. Zemans, Jansing, Nicole L., McClendon, Jazalle, Kage, Hidenori, Sunohara, Mitsuhiro, Alvarez, Juan R., Borok, Zea, Zemans, Rachel L. |
Abstract |
The gas exchange surface of the lungs is lined by an epithelium consisting of alveolar type (AT) I and ATII cells. ATII cells function to produce surfactant, play a role in host defense and fluid and ion transport, and serve as progenitors. ATI cells are important for gas exchange and fluid and ion transport. Our understanding of the biology of these cells depends on the investigation of isolated cells. Here, we present methods for the isolation of mouse and rat ATII cells. |
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