Chapter title |
Application of Metabolomics in Lung Research
|
---|---|
Chapter number | 17 |
Book title |
Lung Innate Immunity and Inflammation
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8570-8_17 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8569-2, 978-1-4939-8570-8
|
Authors |
Nichole A. Reisdorph, Charmion Cruickshank-Quinn, Yasmeen Nkrumah-Elie, Richard Reisdorph, Reisdorph, Nichole A., Cruickshank-Quinn, Charmion, Nkrumah-Elie, Yasmeen, Reisdorph, Richard |
Abstract |
Advancements in omics technologies have increased our potential to evaluate molecular changes in a rapid and comprehensive manner. This is especially true in mass spectrometry-based metabolomics where improvements, including ease of use, in high-performance liquid chromatography (HPLC), column chemistries, instruments, software, and molecular databases, have advanced the field considerably. Applications of this relatively new omics technology in clinical research include discovering disease biomarkers, finding new drug targets, and elucidating disease mechanisms. Here we describe a typical clinical metabolomics workflow, which includes the following steps: (1) extraction of metabolites from the lung, plasma, bronchoalveolar lavage, or cells; (2) sample analysis via liquid chromatography-mass spectrometry; and (3) data analysis using commercial and freely available software packages. Overall, the methods delineated here can help investigators use metabolomics to discovery novel biomarkers and to understand lung diseases. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 1 | 25% |
Student > Doctoral Student | 1 | 25% |
Unknown | 2 | 50% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 1 | 25% |
Unknown | 3 | 75% |