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Lung Innate Immunity and Inflammation

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Cover of 'Lung Innate Immunity and Inflammation'

Table of Contents

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    Book Overview
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    Chapter 1 500 Million Alveoli from 30,000 Feet: A Brief Primer on Lung Anatomy
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    Chapter 2 Overview of Innate Lung Immunity and Inflammation
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    Chapter 3 Isolation and Characterization of Mononuclear Phagocytes in the Mouse Lung and Lymph Nodes
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    Chapter 4 Isolation and Characterization of Mouse Neutrophils
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    Chapter 5 Isolation and Characterization of Mouse Fibroblasts
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    Chapter 6 Isolation of Rat and Mouse Alveolar Type II Epithelial Cells
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    Chapter 7 Isolation and Characterization of Human Alveolar Type II Cells
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    Chapter 8 Air–Liquid Interface Culture of Human and Mouse Airway Epithelial Cells
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    Chapter 9 Isolation and Characterization of Human Lung Myeloid Cells
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    Chapter 10 Measurement of Protein Permeability and Fluid Transport of Human Alveolar Epithelial Type II Cells Under Pathological Conditions
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    Chapter 11 Measuring Innate Immune Function in Mouse Mononuclear Phagocytes
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    Chapter 12 Measuring Neutrophil Bactericidal Activity
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    Chapter 13 Modulation of Myeloid Cell Function Using Conditional and Inducible Transgenic Approaches
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    Chapter 14 Modulation of Lung Epithelial Cell Function Using Conditional and Inducible Transgenic Approaches
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    Chapter 15 Computational Analysis of RNA-Seq Data from Airway Epithelial Cells for Studying Lung Disease
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    Chapter 16 Application of Proteomics in Lung Research
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    Chapter 17 Application of Metabolomics in Lung Research
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    Chapter 18 Functional Genomics in Murine Macrophages
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    Chapter 19 Assessment of Ozone-Induced Lung Injury in Mice
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    Chapter 20 Lung Histological Methods
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    Chapter 21 Intravital Microscopy in the Mouse Lung
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    Chapter 22 Mouse Models of Acute Lung Injury and ARDS
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    Chapter 23 Mouse Models of Asthma
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    Chapter 24 Animal Models of Pulmonary Fibrosis
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    Chapter 25 Mouse Models of COPD
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    Chapter 26 Mouse Models of Viral Infection
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    Chapter 27 Delivery of Therapeutics to the Lung
Attention for Chapter 5: Isolation and Characterization of Mouse Fibroblasts
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Chapter title
Isolation and Characterization of Mouse Fibroblasts
Chapter number 5
Book title
Lung Innate Immunity and Inflammation
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-8570-8_5
Pubmed ID
Book ISBNs
978-1-4939-8569-2, 978-1-4939-8570-8
Authors

Benjamin L. Edelman, Elizabeth F. Redente, Edelman, Benjamin L., Redente, Elizabeth F.

Abstract

The lung parenchyma is comprised of many cells including the structurally important stromal fibroblasts. Fibroblasts function to produce extracellular matrix and are important in the maintenance of alveolar epithelial cells. To understand the role of fibroblasts both in homeostasis and disease, we isolate fibroblasts and grow them in culture. Two methods are presented here for the isolation and maintenance of mouse primary lung fibroblasts.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 43 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 43 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 12 28%
Researcher 6 14%
Student > Doctoral Student 3 7%
Student > Bachelor 3 7%
Student > Master 3 7%
Other 4 9%
Unknown 12 28%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 10 23%
Agricultural and Biological Sciences 7 16%
Medicine and Dentistry 5 12%
Immunology and Microbiology 3 7%
Pharmacology, Toxicology and Pharmaceutical Science 2 5%
Other 4 9%
Unknown 12 28%