Chapter title |
Air–Liquid Interface Culture of Human and Mouse Airway Epithelial Cells
|
---|---|
Chapter number | 8 |
Book title |
Lung Innate Immunity and Inflammation
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8570-8_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8569-2, 978-1-4939-8570-8
|
Authors |
Di Jiang, Niccolette Schaefer, Hong Wei Chu, Jiang, Di, Schaefer, Niccolette, Chu, Hong Wei |
Abstract |
Air-liquid interface culture enables airway epithelial cells to differentiate into a pseudostratified cell layer, consisting of ciliated cells, goblet/secretory cells, and basal cells (Ghio et al., Part Fibre Toxicol 10:25, 2013). This technique is critically important for in vitro studies of lung diseases such as asthma, chronic obstructive pulmonary disease, and cystic fibrosis, since differentiated airway epithelial cells are more representative of the in vivo lung environment than non-differentiated cells (Derichs et al., FASEB J 25:2325-2332, 2011; Hackett et al., Am J Respir Cell Mol Biol 45:1090-1100, 2011;Schneider et al., Am J Respir Crit Care Med 182: 332-340, 2010). Here we describe the process of isolating and expanding human and mouse airway epithelial cells, as well as differentiation of airway epithelial cells by air-liquid interface culture. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 50 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 12 | 24% |
Student > Bachelor | 7 | 14% |
Other | 4 | 8% |
Student > Master | 4 | 8% |
Student > Doctoral Student | 3 | 6% |
Other | 5 | 10% |
Unknown | 15 | 30% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 11 | 22% |
Agricultural and Biological Sciences | 5 | 10% |
Chemistry | 4 | 8% |
Medicine and Dentistry | 4 | 8% |
Engineering | 3 | 6% |
Other | 7 | 14% |
Unknown | 16 | 32% |