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Mouse Oocyte Development

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Attention for Chapter 14: Monitoring Microtubule Dynamics in the Mouse Egg Using Photoactivatable-GFP-Tubulin
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Chapter title
Monitoring Microtubule Dynamics in the Mouse Egg Using Photoactivatable-GFP-Tubulin
Chapter number 14
Book title
Mouse Oocyte Development
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-8603-3_14
Pubmed ID
Book ISBNs
978-1-4939-8602-6, 978-1-4939-8603-3

Greg FitzHarris, FitzHarris, Greg


Fluorescence photoactivation provides a strategy for monitoring protein kinetics within living cells. In particular, fluorescence photoactivation of a subpopulation of microtubule subunits within the spindle using photoactivatable fluorescent tubulin constructs has proven useful for assessing a variety of features of spindle microtubule dynamics, including poleward microtubule movement, microtubule depolymerization, and microtubule turnover, in various cellular settings. The current chapter describes a method for monitoring microtubule dynamics within the mouse egg spindle by photoactivation of photoactivatable-GFP-tubulin, followed by time-lapse confocal imaging.

Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Other 2 50%
Researcher 1 25%
Unknown 1 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 2 50%
Design 1 25%
Unknown 1 25%