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| Chapter title |
Monitoring Microtubule Dynamics in the Mouse Egg Using Photoactivatable-GFP-Tubulin
|
|---|---|
| Chapter number | 14 |
| Book title |
Mouse Oocyte Development
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-8603-3_14 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8602-6, 978-1-4939-8603-3
|
| Authors |
Greg FitzHarris, FitzHarris, Greg |
| Abstract |
Fluorescence photoactivation provides a strategy for monitoring protein kinetics within living cells. In particular, fluorescence photoactivation of a subpopulation of microtubule subunits within the spindle using photoactivatable fluorescent tubulin constructs has proven useful for assessing a variety of features of spindle microtubule dynamics, including poleward microtubule movement, microtubule depolymerization, and microtubule turnover, in various cellular settings. The current chapter describes a method for monitoring microtubule dynamics within the mouse egg spindle by photoactivation of photoactivatable-GFP-tubulin, followed by time-lapse confocal imaging. |
Mendeley readers
The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 4 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Other | 2 | 50% |
| Researcher | 1 | 25% |
| Unknown | 1 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 2 | 50% |
| Design | 1 | 25% |
| Unknown | 1 | 25% |