Micropipette Aspiration of Oocytes to Assess Cortical Tension
Mouse Oocyte Development
Methods in molecular biology, January 2018
Janice P. Evans, Douglas N. Robinson, Evans, Janice P., Robinson, Douglas N.
Just as it is important to understand the cell biology of signaling pathways, it is valuable also to understand mechanical forces in cells. The field of mechanobiology has a rich history, including study of cellular mechanics during mitosis and meiosis in echinoderm oocytes and zygotes dating back to the 1930s. This chapter addresses the use of micropipette aspiration (MPA) to assess cellular mechanics, specifically cortical tension, in mammalian oocytes.
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