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Mouse Oocyte Development

Overview of attention for book
Cover of 'Mouse Oocyte Development'

Table of Contents

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    Book Overview
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    Chapter 1 Growth In Vitro of Granulosa Cell–Oocyte Complexes of the Mouse
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    Chapter 2 In Vitro Culture of Mouse Oocytes for Meiotic Maturation
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    Chapter 3 In Vitro Maturation and Culture of Human Oocytes
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    Chapter 4 Pronuclear Transfer in Human Oocytes
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    Chapter 5 In Vitro Fertilization of Mouse Oocyte
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    Chapter 6 Profiling Maternal mRNA Translation During Oocyte Development
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    Chapter 7 Transcriptome Profiling of Single Mouse Oocytes
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    Chapter 8 Immunofluorescence Technique to Detect Subcellular Structures Critical to Oocyte Maturation
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    Chapter 9 Immunofluorescence Staining of K-Fibers in Mouse Oocytes Using Cold Fixation
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    Chapter 10 Triple-Color Live Imaging of Mouse Oocytes
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    Chapter 11 Detection of Separase Activity Using a Cleavage Sensor in Live Mouse Oocytes
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    Chapter 12 Manipulating Cohesin Levels in Live Mouse Oocytes
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    Chapter 13 Optogenetic Manipulation of Mouse Oocytes
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    Chapter 14 Monitoring Microtubule Dynamics in the Mouse Egg Using Photoactivatable-GFP-Tubulin
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    Chapter 15 Photoactivation of Actin in Mouse Oocyte
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    Chapter 16 Laser Ablation of Microtubule–Chromosome Attachment in Mouse Oocytes
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    Chapter 17 Micropipette Aspiration of Oocytes to Assess Cortical Tension
Attention for Chapter 10: Triple-Color Live Imaging of Mouse Oocytes
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Chapter title
Triple-Color Live Imaging of Mouse Oocytes
Chapter number 10
Book title
Mouse Oocyte Development
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-8603-3_10
Pubmed ID
29961258
Book ISBNs
978-1-4939-8602-6, 978-1-4939-8603-3
Authors

Aurélien Courtois, Petr Solc, Tomoya S. Kitajima, Courtois, Aurélien, Solc, Petr, Kitajima, Tomoya S.

Abstract

Meiotic division is a dynamic process that exhibits active interactive behaviors amongst different intracellular structures and components for spindle assembly and chromosome segregation. Understanding the mechanisms of meiotic spindle assembly and chromosome segregation therefore requires a quantitative analysis of spatiotemporal relationships among different structures and components. In this chapter, we describe a method for triple-color live imaging of meiotic division in mouse oocytes. This approach combines the microinjection of RNAs encoding proteins tagged with green and red fluorescent proteins and the visualization of microtubules with the fluorogenic far-red probe SiR-Tubulin. This method enables the simultaneous spatiotemporal mapping of three different components of the spindle and chromosomes, which opens the way to quantitative analysis of their interactive behaviors.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 2 40%
Student > Ph. D. Student 1 20%
Professor > Associate Professor 1 20%
Student > Postgraduate 1 20%
Readers by discipline Count As %
Agricultural and Biological Sciences 3 60%
Biochemistry, Genetics and Molecular Biology 2 40%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 July 2018.
All research outputs
#20,523,725
of 23,094,276 outputs
Outputs from Methods in molecular biology
#9,976
of 13,207 outputs
Outputs of similar age
#378,481
of 442,643 outputs
Outputs of similar age from Methods in molecular biology
#1,194
of 1,499 outputs
Altmetric has tracked 23,094,276 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,207 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 442,643 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,499 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.

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