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Mouse Oocyte Development

Overview of attention for book
Attention for Chapter 10: Triple-Color Live Imaging of Mouse Oocytes
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Chapter title
Triple-Color Live Imaging of Mouse Oocytes
Chapter number 10
Book title
Mouse Oocyte Development
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-8603-3_10
Pubmed ID
Book ISBNs
978-1-4939-8602-6, 978-1-4939-8603-3
Authors

Aurélien Courtois, Petr Solc, Tomoya S. Kitajima, Courtois, Aurélien, Solc, Petr, Kitajima, Tomoya S.

Abstract

Meiotic division is a dynamic process that exhibits active interactive behaviors amongst different intracellular structures and components for spindle assembly and chromosome segregation. Understanding the mechanisms of meiotic spindle assembly and chromosome segregation therefore requires a quantitative analysis of spatiotemporal relationships among different structures and components. In this chapter, we describe a method for triple-color live imaging of meiotic division in mouse oocytes. This approach combines the microinjection of RNAs encoding proteins tagged with green and red fluorescent proteins and the visualization of microtubules with the fluorogenic far-red probe SiR-Tubulin. This method enables the simultaneous spatiotemporal mapping of three different components of the spindle and chromosomes, which opens the way to quantitative analysis of their interactive behaviors.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 25%
Professor > Associate Professor 1 25%
Student > Bachelor 1 25%
Student > Postgraduate 1 25%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 50%
Agricultural and Biological Sciences 2 50%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 July 2018.
All research outputs
#11,697,431
of 13,171,980 outputs
Outputs from Methods in molecular biology
#6,005
of 8,463 outputs
Outputs of similar age
#233,023
of 268,353 outputs
Outputs of similar age from Methods in molecular biology
#1
of 1 outputs
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So far Altmetric has tracked 8,463 research outputs from this source. They receive a mean Attention Score of 2.1. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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