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Mouse Oocyte Development

Overview of attention for book
Attention for Chapter 12: Manipulating Cohesin Levels in Live Mouse Oocytes
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  • Above-average Attention Score compared to outputs of the same age (54th percentile)

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6 tweeters

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15 Mendeley
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Chapter title
Manipulating Cohesin Levels in Live Mouse Oocytes
Chapter number 12
Book title
Mouse Oocyte Development
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-8603-3_12
Pubmed ID
Book ISBNs
978-1-4939-8602-6, 978-1-4939-8603-3
Authors

Anna Szydłowska, Sabrina Ladstätter, Kikuë Tachibana, Szydłowska, Anna, Ladstätter, Sabrina, Tachibana, Kikuë

Abstract

The cohesin complex is essential for chromosome segregation in mitosis and meiosis. Cohesin is a tripartite protein complex that holds sister chromatids together from DNA replication until anaphase. In mammals, meiotic DNA replication occurs in oogonia of embryos and chromosome segregation occurs in oocytes of sexually mature females. Sister chromatid cohesion establishment and chromosome segregation are thus separated by months in the mouse and decades in the human. The meiotic cohesin complex that maintains sister chromatid cohesion must therefore hold replicated sisters together for a long time in oocytes. Remarkably, this is achieved by establishing cohesion exclusively in prenatal oocytes. Meiotic cohesion in females is maintained without detectable turnover and cohesin is therefore thought to be a long-lived protein complex. Nevertheless, the lifespan of cohesin molecules is limited as chromosomal cohesin levels decline with maternal age. The age-related loss of cohesin and weakened cohesion correlate with an age-related increase in chromosome missegregation of meiosis I oocytes that results in aneuploid eggs. Therefore, loss of chromosomal cohesin has been proposed to be a leading cause of the maternal age effect. To better understand cohesin deterioration in oocytes, it is crucial to gain insights into mammalian cohesion establishment and maintenance mechanisms by manipulating cohesin in live oocytes.This chapter describes techniques that address the manipulation of meiotic cohesin levels in mouse oocytes. First, we describe how cohesin can be efficiently removed from meiotic chromosomes by injecting mRNA encoding TEV protease in live oocytes expressing cohesin with engineered TEV recognition sites, followed by imaging. Secondly, we describe how cohesin expression can be induced during different stages of oocyte development using genetically modified mouse strains. In particular, we describe how to determine the deletion timing of germline-specific Cre recombinases using β-galactosidase staining of fetal ovaries. Lastly, we provide guidance on how to quantify cohesin levels on metaphase I chromosome spreads.

Twitter Demographics

The data shown below were collected from the profiles of 6 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 15 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 3 20%
Student > Ph. D. Student 3 20%
Professor > Associate Professor 2 13%
Student > Doctoral Student 1 7%
Librarian 1 7%
Other 2 13%
Unknown 3 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 8 53%
Agricultural and Biological Sciences 2 13%
Medicine and Dentistry 1 7%
Unknown 4 27%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 July 2018.
All research outputs
#11,764,646
of 21,192,559 outputs
Outputs from Methods in molecular biology
#2,812
of 11,944 outputs
Outputs of similar age
#133,226
of 296,919 outputs
Outputs of similar age from Methods in molecular biology
#1
of 1 outputs
Altmetric has tracked 21,192,559 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 11,944 research outputs from this source. They receive a mean Attention Score of 3.3. This one has done well, scoring higher than 76% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 296,919 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 54% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them