| Chapter title |
Profiling Maternal mRNA Translation During Oocyte Development
|
|---|---|
| Chapter number | 6 |
| Book title |
Mouse Oocyte Development
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-8603-3_6 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8602-6, 978-1-4939-8603-3
|
| Authors |
Joao P. Sousa Martins, Marco Conti, Martins, Joao P. Sousa, Conti, Marco |
| Abstract |
With the progress in our understanding of germ cell development, there is an emerging need to investigate the mechanisms of mRNA translation functioning in these cells. Indeed, posttranscriptional regulations of gene expression drive the most important transitions of the germ cell life cycle. Here we describe a strategy to measure mRNA translation in the oocyte, taking advantage of an approach originally developed to identify the transcriptome of a subgroup of cells in a complex cell mixture. This technique takes advantage of the "RiboTag" approach to express an HA-tag on the large ribosomal subunit of the ribosomes in the oocyte. Immunoprecipitation of the extracts followed by qPCR or RNAseq is used to identify mRNAs actively translated. |
X Demographics
As of 1 July 2024, you may notice a temporary increase in the numbers of X profiles with Unknown location. Click here to learn more.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 20% |
| United States | 1 | 20% |
| Unknown | 3 | 60% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 5 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 17 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Doctoral Student | 4 | 24% |
| Student > Ph. D. Student | 3 | 18% |
| Other | 2 | 12% |
| Student > Master | 1 | 6% |
| Professor > Associate Professor | 1 | 6% |
| Other | 1 | 6% |
| Unknown | 5 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 6 | 35% |
| Agricultural and Biological Sciences | 4 | 24% |
| Medicine and Dentistry | 2 | 12% |
| Neuroscience | 1 | 6% |
| Unknown | 4 | 24% |