Report for: Mouse Oocyte Development

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Chapter title	Photoactivation of Actin in Mouse Oocyte
Chapter number	15
Book title	Mouse Oocyte Development
Published in	Methods in molecular biology, January 2018
DOI	10.1007/978-1-4939-8603-3_15
Pubmed ID	29961263
Book ISBNs	978-1-4939-8602-6, 978-1-4939-8603-3
Authors	Maria Almonacid, Almonacid, Maria
Abstract	Development of fluorescence distribution assays like FRAP (fluorescence recovery after photobleaching) or photoactivation has had a great impact in studying intracellular protein dynamics. In particular, the cytoskeleton field largely benefited from these techniques, with lots of new information provided about the dynamics and organization of actin networks whithin cells.In mouse oocyte, actin photoactivation has been very useful to determine the dynamics of different actin structures involved in meiotic divisions, including a cytoplasmic meshwork and a subcortical actin layer.Here, we describe a method, actin photoactivation, to determine the dynamics of the actin cytoplasmic meshwork and the subcortical actin layer during the first meiotic division in the mouse oocyte, that could be adapted to other actin structures or other stages of meiotic divisions.

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Mouse Oocyte Development

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