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Cell Cycle Oscillators

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Cover of 'Cell Cycle Oscillators'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Cell Cycle Control: A System of Interlinking Oscillators.
  3. Altmetric Badge
    Chapter 2 Cell Cycle Oscillators
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    Chapter 3 Role of Computational Modeling in Understanding Cell Cycle Oscillators
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    Chapter 4 E2F Transcription Factors Control the Roller Coaster Ride of Cell Cycle Gene Expression
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    Chapter 5 Cell Synchronization of Mouse Embryonic Fibroblasts
  7. Altmetric Badge
    Chapter 6 Cell Cycle Oscillators
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    Chapter 7 Elutriation for Cell Cycle Synchronization in Fission Yeast.
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    Chapter 8 Spatiotemporal Investigation of Phosphorylation Events During Cell Cycle Progression
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    Chapter 9 Cell Cycle Dynamics of Proteins and Post-translational Modifications Using Quantitative Immunofluorescence
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    Chapter 10 Building a Synthetic Transcriptional Oscillator.
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    Chapter 11 Cell Cycle Oscillators
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    Chapter 12 A Computational Method for Identifying Yeast Cell Cycle Transcription Factors.
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    Chapter 13 Measuring Activity and Specificity of Protein Phosphatases
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    Chapter 14 Combining the Optimized Yeast Cytosine Deaminase Protein Fragment Complementation Assay and an In Vitro Cdk1 Targeting Assay to Study the Regulation of the γ-Tubulin Complex.
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    Chapter 15 Cell Cycle Synchronization Using a Microfluidic Synchronizer for Fission Yeast Cells.
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    Chapter 16 Detection of Protein-Protein Interactions in Tobacco BY-2 Cells Using Bimolecular Fluorescence Complementation.
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    Chapter 17 Tracking the Cyclin B1-GFP Sensor to Profile the Pattern of Mitosis Versus Mitotic Bypass
  19. Altmetric Badge
    Chapter 18 Cell Cycle Oscillators
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    Chapter 19 Using the Fly-FUCCI System for the Live Analysis of Cell Cycle Dynamics in Cultured Drosophila Cells.
  21. Altmetric Badge
    Chapter 20 Imaging Cell Cycle Phases and Transitions of Living Cells from Yeast to Woman.
  22. Altmetric Badge
    Chapter 21 Measurement of Cdk1/Cyclin B Kinase Activity by Specific Antibodies and Western Blotting
  23. Altmetric Badge
    Chapter 22 Cell Cycle Oscillators
Attention for Chapter 7: Elutriation for Cell Cycle Synchronization in Fission Yeast.
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Chapter title
Elutriation for Cell Cycle Synchronization in Fission Yeast.
Chapter number 7
Book title
Cell Cycle Oscillators
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-2957-3_7
Pubmed ID
Book ISBNs
978-1-4939-2956-6, 978-1-4939-2957-3
Authors

Kume, Kazunori, Kazunori Kume

Abstract

Cell synchronization is a powerful technique for studying the eukaryotic cell cycle events precisely. The fission yeast is a rod-shaped cell whose growth is coordinated with the cell cycle. Monitoring the cellular growth of fission yeast is a relatively simple way to measure the cell cycle stage of a cell. Here, we describe a detailed method of unperturbed cell synchronization, named centrifugal elutriation, for fission yeast.

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