Chapter title |
Building a Synthetic Transcriptional Oscillator.
|
---|---|
Chapter number | 10 |
Book title |
Cell Cycle Oscillators
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-2957-3_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2956-6, 978-1-4939-2957-3
|
Authors |
Schwarz-Schilling, Matthaeus, Kim, Jongmin, Cuba, Christian, Weitz, Maximilian, Franco, Elisa, Simmel, Friedrich C, Matthaeus Schwarz-Schilling, Jongmin Kim, Christian Cuba, Maximilian Weitz, Elisa Franco, Friedrich C. Simmel |
Abstract |
Reaction circuits mimicking genetic oscillators can be realized with synthetic, switchable DNA genes (so-called genelets), and two enzymes only, an RNA polymerase and a ribonuclease. The oscillatory behavior of the genelets is driven by the periodic production and degradation of RNA effector molecules. Here, we describe the preparation, assembly, and testing of a synthetic, transcriptional two-node negative-feedback oscillator, whose dynamics can be followed in real-time by fluorescence read-out. |
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