| Chapter title |
Measurement of Cdk1/Cyclin B Kinase Activity by Specific Antibodies and Western Blotting
|
|---|---|
| Chapter number | 21 |
| Book title |
Cell Cycle Oscillators
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-2957-3_21 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2956-6, 978-1-4939-2957-3
|
| Authors |
Cody W. Lewis, Ryan G. Taylor, Roy M. Golsteyn |
| Abstract |
Quantitative measurement of enzyme activity is a valuable approach to study how cells function. We present a method to measure the activity of the enzyme Cdk1/cyclin B. This enzyme is required by all eukaryotic cells to enter mitosis. Therefore, a biochemical assay to measure Cdk1/cyclin B activity can be used to identify cell populations that are in mitosis or to detect inhibitors of Cdk1/Cyclin B in vitro. A key distinction of the method presented here, compared to others, is that it uses a recombinant protein, a specific antibody, and a western blot apparatus, which makes the technique available to cell and molecular biology laboratories who do not wish to use radioisotopes, which are commonly required for other protein kinase assays. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 2 | 29% |
| Researcher | 2 | 29% |
| Student > Ph. D. Student | 1 | 14% |
| Unspecified | 1 | 14% |
| Student > Master | 1 | 14% |
| Other | 0 | 0% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 3 | 43% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 14% |
| Unspecified | 1 | 14% |
| Biochemistry, Genetics and Molecular Biology | 1 | 14% |
| Neuroscience | 1 | 14% |
| Other | 0 | 0% |