| Chapter title |
Cell Synchronization of Mouse Embryonic Fibroblasts
|
|---|---|
| Chapter number | 5 |
| Book title |
Cell Cycle Oscillators
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-2957-3_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2956-6, 978-1-4939-2957-3
|
| Authors |
Michael J. Thwaites, Courtney H. Coschi, Christian E. Isaac, Frederick A. Dick |
| Abstract |
A fundamental need in the analysis of the cell cycle is the ability to isolate relatively homogeneous populations of cells in different phases. This is complicated by the variable proliferative properties and responses to synchronizing methods of different cancer-derived cell lines. Paradoxically, cell lines with genetic defects in cell cycle control are sometimes chosen because they are amenable to chemical synchronization. Embryonic fibroblasts from mice present the opportunity to study the effects of defined genetic modifications on a normal cell cycle. However, synchronization of these cells has often been challenging. In this chapter we outline three basic protocols for isolating mouse fibroblasts at the G1-to-S-phase transition, in S phase, and during mitosis. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 16 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 6 | 38% |
| Researcher | 2 | 13% |
| Student > Master | 2 | 13% |
| Professor | 1 | 6% |
| Unspecified | 1 | 6% |
| Other | 1 | 6% |
| Unknown | 3 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 7 | 44% |
| Computer Science | 2 | 13% |
| Unspecified | 1 | 6% |
| Agricultural and Biological Sciences | 1 | 6% |
| Medicine and Dentistry | 1 | 6% |
| Other | 0 | 0% |
| Unknown | 4 | 25% |