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Mendeley readers
Chapter title |
The Synthesis of Designer DNA
|
---|---|
Chapter number | 2 |
Book title |
DNA Nanotechnology
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8582-1_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8581-4, 978-1-4939-8582-1
|
Authors |
Rachel C. Little, Colette J. Whitfield, Eimer M. Tuite, Andrew R. Pike |
Abstract |
The synthesis of designer DNA requires an approach where the user can determine both the sequence and the number of nucleobases. The protocol outlined here describes an enzymatic method for the synthesis of long repeat-sequence DNA. The method utilizes a PCR-based approach; starting with short oligo-seeds, c.a. 20 bp, bearing a minimum of two repeating units of >8 bp sequences. During each heat-cool cycle, the oligo-seeds reanneal imperfectly leaving an overhang, which is then extended by the polymerase. The final length of the DNA is determined by the number of heat-cool cycles performed, reaching up to 20,000 bp after 20 cycles. |
Mendeley readers
The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 2 | 29% |
Lecturer > Senior Lecturer | 1 | 14% |
Student > Master | 1 | 14% |
Unknown | 3 | 43% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 1 | 14% |
Agricultural and Biological Sciences | 1 | 14% |
Materials Science | 1 | 14% |
Chemistry | 1 | 14% |
Unknown | 3 | 43% |