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Timeline
Mendeley readers
| Chapter title |
Selection of Structure-Switching DNA Aptamers Binding Soluble Small Molecules and SPR Validation of Enrichment
|
|---|---|
| Chapter number | 13 |
| Book title |
DNA Nanotechnology
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-8582-1_13 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8581-4, 978-1-4939-8582-1
|
| Authors |
Enrico Tenaglia, Carlotta Guiducci, Fabio M. Spiga |
| Abstract |
Capture-SELEX is an effective molecular strategy enabling the discovery of structure-switching aptamers, which might find useful application in molecular detection or separation. We here provide a protocol to perform capture-SELEX for DNA aptamers binding soluble small molecules, which includes a straightforward functional validation by SPR. The SELEX strategy here described is adaptable to any water-soluble molecular target and might foster the development of DNA aptamers binding therapeutic small molecules, at the great advantage of clinical bioanalytics. |
Mendeley readers
The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 14 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 3 | 21% |
| Student > Ph. D. Student | 2 | 14% |
| Researcher | 2 | 14% |
| Student > Master | 1 | 7% |
| Professor | 1 | 7% |
| Other | 0 | 0% |
| Unknown | 5 | 36% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 29% |
| Chemistry | 2 | 14% |
| Immunology and Microbiology | 1 | 7% |
| Agricultural and Biological Sciences | 1 | 7% |
| Unknown | 6 | 43% |