| Chapter title |
A Flowchart to Analyze Protease Activity in Plant Mitochondria.
|
|---|---|
| Chapter number | 8 |
| Book title |
Plant Mitochondria
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2639-8_8 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2638-1, 978-1-4939-2639-8
|
| Authors |
Teixeira, Pedro F, Branca, Rui M, Kmiec, Beata, Glaser, Elzbieta, Teixeira, Pedro F., Branca, Rui M., Pedro F. Teixeira, Rui M. Branca, Beata Kmiec, Elzbieta Glaser |
| Editors |
Whelan, James, Murcha, Monika W. |
| Abstract |
Proteases are one of the most abundant classes of enzymes and are involved in a plethora of biological processes in many cellular compartments, including the mitochondria. To understand the role of proteases is essential to determine their substrate repertoire, preferably in an in vivo setting. In this chapter we describe general guidelines to analyze protease activity using several strategies, from in-gel analysis to mass spectrometry mapping of the cleavage site(s) and fluorogenic probes that can easily be used in vivo. To exemplify this flowchart, we used the recently characterized organellar oligopeptidase of Arabidopsis (Arabidopsis thaliana), an enzyme that takes part in degradation of short peptides within mitochondria and chloroplasts. |
Mendeley readers
Geographical breakdown
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|---|---|---|
| Unknown | 4 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 1 | 25% |
| Other | 1 | 25% |
| Unknown | 2 | 50% |
| Readers by discipline | Count | As % |
|---|---|---|
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| Unknown | 2 | 50% |