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Plant Mitochondria

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Cover of 'Plant Mitochondria'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Isolation of Intact Mitochondria from the Model Plant Species Arabidopsis thaliana and Oryza sativa
  3. Altmetric Badge
    Chapter 2 Determining Mitochondrial Transcript Termini for the Study of Transcription Start Sites and Transcript 5′ End Maturation
  4. Altmetric Badge
    Chapter 3 Mitochondrial Run-On Transcription Assay Using Biotin Labeling.
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    Chapter 4 In Vitro RNA Uptake Studies in Plant Mitochondria
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    Chapter 5 In Vitro and In Vivo Protein Uptake Studies in Plant Mitochondria
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    Chapter 6 Plant Mitochondrial Proteomics
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    Chapter 7 Identification of Lysine-Acetylated Mitochondrial Proteins and Their Acetylation Sites
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    Chapter 8 A Flowchart to Analyze Protease Activity in Plant Mitochondria.
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    Chapter 9 Activity Measurements of Mitochondrial Enzymes in Native Gels
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    Chapter 10 Activity Assay for Plant Mitochondrial Enzymes
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    Chapter 11 Analysis of Type II NAD(P)H Dehydrogenases
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    Chapter 12 Assessment of Respiration in Isolated Plant Mitochondria Using Clark-Type Electrodes
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    Chapter 13 Micro-Respiratory Measurements in Plants.
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    Chapter 14 Improvements to Define Mitochondrial Metabolomics Using Nonaqueous Fractionation.
  16. Altmetric Badge
    Chapter 15 Plant Mitochondria
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    Chapter 16 Imaging and Analysis of Mitochondrial Dynamics in Living Cells
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    Chapter 17 Analysis of Plant Mitochondrial Function Using Fluorescent Protein Sensors.
  19. Altmetric Badge
    Chapter 18 In Planta Analysis of Leaf Mitochondrial Superoxide and Nitric Oxide
  20. Altmetric Badge
    Chapter 19 Databases and Informatics Resources for Analysis of Plant Mitochondria
  21. Altmetric Badge
    Chapter 20 Expression and Crystallization of the Plant Alternative Oxidase.
Attention for Chapter 17: Analysis of Plant Mitochondrial Function Using Fluorescent Protein Sensors.
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Chapter title
Analysis of Plant Mitochondrial Function Using Fluorescent Protein Sensors.
Chapter number 17
Book title
Plant Mitochondria
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2639-8_17
Pubmed ID
Book ISBNs
978-1-4939-2638-1, 978-1-4939-2639-8
Authors

Wagner, Stephan, Nietzel, Thomas, Aller, Isabel, Costa, Alex, Fricker, Mark D, Meyer, Andreas J, Schwarzländer, Markus, Stephan Wagner, Thomas Nietzel, Isabel Aller, Alex Costa, Mark D. Fricker, Andreas J. Meyer, Markus Schwarzländer

Editors

James Whelan, Monika W. Murcha

Abstract

Mitochondrial physiology sets the basis for function of the organelle and vice versa. While a limited range of in vivo parameters, such as oxygen consumption, has been classically accessible for measurement, a growing collection of fluorescent protein sensors can now give insights into the physiology of plant mitochondria. Nevertheless, the meaningful application of these sensors in mitochondria is technically challenging and requires rigorous experimental standards. Here we exemplify the application of three genetically encoded sensors to monitor glutathione redox potential, pH, and calcium in the matrix of mitochondria in intact plants. We describe current methods for quantitative imaging and analysis in living root tips by confocal microscopy and discuss methodological limitations.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 25 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 25 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 24%
Student > Master 5 20%
Student > Ph. D. Student 5 20%
Student > Bachelor 2 8%
Student > Doctoral Student 2 8%
Other 3 12%
Unknown 2 8%
Readers by discipline Count As %
Agricultural and Biological Sciences 12 48%
Biochemistry, Genetics and Molecular Biology 9 36%
Design 1 4%
Unknown 3 12%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 January 2016.
All research outputs
#20,302,535
of 22,840,638 outputs
Outputs from Methods in molecular biology
#9,917
of 13,127 outputs
Outputs of similar age
#295,932
of 353,212 outputs
Outputs of similar age from Methods in molecular biology
#636
of 997 outputs
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So far Altmetric has tracked 13,127 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 997 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.