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Chapter title |
In Planta Analysis of Leaf Mitochondrial Superoxide and Nitric Oxide
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Chapter number | 18 |
Book title |
Plant Mitochondria
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Published in |
Methods in molecular biology, January 2015
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DOI | 10.1007/978-1-4939-2639-8_18 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2638-1, 978-1-4939-2639-8
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Authors |
Marina Cvetkovska, Greg C. Vanlerberghe |
Editors |
James Whelan, Monika W. Murcha |
Abstract |
Superoxide (O2 (-)) and nitric oxide (NO) are produced within plant mitochondria and may have signaling functions within the cell. Here we describe semiquantitative fluorescence imaging-based approaches to estimate the mitochondrial amount of these reactive and short-lived species within intact leaf tissue. We also outline a biochemical method using oxyhemoglobin to measure NO within a whole leaf tissue extract. This quantitative method, while not specifically evaluating mitochondrial localized NO, does nonetheless provide another independent measure of NO that can be useful. |
Mendeley readers
The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 2 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Doctoral Student | 1 | 50% |
Student > Master | 1 | 50% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 1 | 50% |
Medicine and Dentistry | 1 | 50% |