| Chapter title |
Plant Mitochondrial Proteomics
|
|---|---|
| Chapter number | 6 |
| Book title |
Plant Mitochondria
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2639-8_6 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2638-1, 978-1-4939-2639-8
|
| Authors |
Nicolas L. Taylor, A. Harvey Millar |
| Editors |
James Whelan, Monika W. Murcha |
| Abstract |
Mitochondrial proteomics has significantly developed since the first plant mitochondrial proteomes were published in 2001. Many studies have added to our knowledge of the protein components that make up plant mitochondria in a wide range of species. Here we present two common and one emerging quantitative proteomic techniques that can be used to study the abundance of mitochondrial proteins. For this publication, we have described the methods as an approach to determine the amount of contamination in a mitochondrial isolation to contrast historical approaches that involved the use of use of antibodies to specific marker proteins or the measurement of activity of marker enzymes. However, these approaches could easily be adapted to carry out control versus treatment studies. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 11 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 3 | 27% |
| Student > Master | 2 | 18% |
| Professor > Associate Professor | 2 | 18% |
| Researcher | 1 | 9% |
| Professor | 1 | 9% |
| Other | 0 | 0% |
| Unknown | 2 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 4 | 36% |
| Biochemistry, Genetics and Molecular Biology | 4 | 36% |
| Arts and Humanities | 1 | 9% |
| Unknown | 2 | 18% |