| Chapter title |
Determining Mitochondrial Transcript Termini for the Study of Transcription Start Sites and Transcript 5′ End Maturation
|
|---|---|
| Chapter number | 2 |
| Book title |
Plant Mitochondria
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2639-8_2 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2638-1, 978-1-4939-2639-8
|
| Authors |
Stefan Binder, Kristina Kühn |
| Editors |
James Whelan, Monika W. Murcha |
| Abstract |
Mitochondrial gene expression in plants is considerably more complex than in animals or fungi. In plants, mitochondrial transcripts are generated from transcription initiation at numerous, poorly conserved promoters located throughout the mitochondrial genome. Most genes have more than one transcription start site. Posttranscriptional RNA 5' end maturation contributes to the diversity of transcripts produced from each mitochondrial gene. Understanding transcriptional mechanisms and transcript maturation requires knowledge on transcription start sites and processing sites. This chapter describes two different, complementary experimental approaches for determining these sites in mitochondrial genomes through mapping of transcript 5' ends. In order to distinguish 5' ends deriving from transcription initiation, both strategies exploit the presence of triphosphates at these specific 5' termini. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 5 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 1 | 20% |
| Researcher | 1 | 20% |
| Other | 1 | 20% |
| Student > Doctoral Student | 1 | 20% |
| Unknown | 1 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 60% |
| Agricultural and Biological Sciences | 2 | 40% |