| Chapter title |
Amyloid-binding proteins: affinity-based separation, proteomic identification, and optical biosensor validation.
|
|---|---|
| Chapter number | 33 |
| Book title |
Proteomic Profiling
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2550-6_33 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2549-0, 978-1-4939-2550-6
|
| Authors |
Medvedev, Alexei, Buneeva, Olga, Kopylov, Arthur, Gnedenko, Oksana, Ivanov, Alexis, Zgoda, Victor, Makarov, Alexander A, Alexei Medvedev, Olga Buneeva, Arthur Kopylov, Oksana Gnedenko, Alexis Ivanov, Victor Zgoda, Alexander A. Makarov |
| Abstract |
The amyloid-beta peptide is considered as a key player in the development and progression of Alzheimer's disease (AD). Although good evidence exists that amyloid-beta accumulates inside cells, intracellular brain amyloid-beta-binding proteins remain poorly characterized. Here we describe a protocol for affinity-based profiling of amyloid-beta-binding proteins of rat brain, their proteomic identification and validation by a surface plasmon resonance (SPR)-based analysis. It includes: (a) SPR-based selection of immobilization conditions for beta-amyloid coupling and choice of appropriate resin for preparation of an affinity sorbent; (b) immobilization of beta-amyloid on the selected resin; |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 13 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 23% |
| Student > Bachelor | 2 | 15% |
| Student > Master | 1 | 8% |
| Unknown | 7 | 54% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 23% |
| Agricultural and Biological Sciences | 1 | 8% |
| Medicine and Dentistry | 1 | 8% |
| Unknown | 8 | 62% |