| Chapter title |
Proteomic Profiling
|
|---|---|
| Chapter number | 27 |
| Book title |
Proteomic Profiling
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2550-6_27 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2549-0, 978-1-4939-2550-6
|
| Authors |
Gilda, Jennifer E, Gomes, Aldrin V, Jennifer E. Gilda, Aldrin V. Gomes, Gilda, Jennifer E., Gomes, Aldrin V. |
| Abstract |
Western blotting is a commonly used laboratory technique for semi-quantifying protein amounts. It is important when quantifying protein expression to account for differences in the amount of total protein loaded onto the gel using a loading control. Common loading controls include housekeeping proteins, such as β-actin or GAPDH, quantified by Western blot, or total protein, quantified using a stain such as Coomassie Brilliant Blue or Ponceau S. A more recently developed method for total protein quantification utilizes stain-free technology, which has a linear dynamic detection range and allows for protein detection on both gels and membranes. Here, we describe the theory and use of stain-free gels for total protein quantification and normalization of Western blots. |
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