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Mendeley readers
Chapter title |
Optimization of Cell Lysis and Protein Digestion Protocols for Protein Analysis by LC-MS/MS
|
---|---|
Chapter number | 20 |
Book title |
Proteomic Profiling
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2550-6_20 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2549-0, 978-1-4939-2550-6
|
Authors |
Dominic Winter, Alireza Dehghani, Hanno Steen |
Abstract |
Mass spectrometry-based proteomics is the method of choice for analyzing (sub-) proteomes from virtually any source; with cells grown in culture being the most frequently investigated samples. Using HeLa cells, we describe a strategy for the optimization of protocols for whole proteome analysis. We cover cell lysis, protein precipitation and digestion, comparing the results obtained using different parameters and offering various possibilities for the optimization of a proteomic workflow which can be used for virtually any type of animal cell grown in culture. |
Mendeley readers
The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Germany | 1 | 4% |
Unknown | 25 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 10 | 38% |
Student > Master | 3 | 12% |
Researcher | 3 | 12% |
Other | 2 | 8% |
Professor | 1 | 4% |
Other | 2 | 8% |
Unknown | 5 | 19% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 6 | 23% |
Agricultural and Biological Sciences | 4 | 15% |
Neuroscience | 2 | 8% |
Engineering | 2 | 8% |
Medicine and Dentistry | 2 | 8% |
Other | 4 | 15% |
Unknown | 6 | 23% |