Chapter title |
Dynamic Range Compression with ProteoMiner™: Principles and Examples
|
---|---|
Chapter number | 9 |
Book title |
Proteomic Profiling
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2550-6_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2549-0, 978-1-4939-2550-6
|
Authors |
Lei Li |
Abstract |
One of the main challenges in proteomics investigation, protein biomarker research, and protein purity and contamination analysis is how to efficiently enrich and detect low-abundance proteins in biological samples. One approach that makes the detection of rare species possible is the treatment of biological samples with solid-phase combinatorial peptide ligand libraries, ProteoMiner. This method utilizes hexapeptide bead library with huge diversity to bind and enrich low-abundance proteins but remove most of the high-abundance proteins, therefore compresses the protein abundance range in the samples. This work describes optimized protocols and highlights on the successful application of ProteoMiner to protein identification and analysis. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 14 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 5 | 36% |
Student > Ph. D. Student | 3 | 21% |
Student > Doctoral Student | 1 | 7% |
Student > Master | 1 | 7% |
Researcher | 1 | 7% |
Other | 0 | 0% |
Unknown | 3 | 21% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 6 | 43% |
Biochemistry, Genetics and Molecular Biology | 3 | 21% |
Unknown | 5 | 36% |