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Timeline
Mendeley readers
Chapter title |
Mitochondrial Membrane Potential Assay
|
---|---|
Chapter number | 2 |
Book title |
High-Throughput Screening Assays in Toxicology
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-6346-1_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6344-7, 978-1-4939-6346-1
|
Authors |
Srilatha Sakamuru, Matias S. Attene-Ramos, Menghang Xia, Sakamuru, Srilatha, Attene-Ramos, Matias S., Xia, Menghang |
Abstract |
Mitochondrial function, a key indicator of cell health, can be assessed by monitoring changes in mitochondrial membrane potential (MMP). Cationic fluorescent dyes are commonly used tools to assess MMP. We used a water-soluble mitochondrial membrane potential indicator (m-MPI) to detect changes in MMP in HepG2 cells. A homogenous cell-based MMP assay was optimized and performed in a 1536-well plate format to screen several compound libraries for mitochondrial toxicity by evaluating the effects of chemical compounds on MMP. |
Mendeley readers
The data shown below were compiled from readership statistics for 283 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Portugal | 3 | 1% |
Germany | 1 | <1% |
South Africa | 1 | <1% |
United Kingdom | 1 | <1% |
Poland | 1 | <1% |
Unknown | 276 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 75 | 27% |
Researcher | 33 | 12% |
Student > Bachelor | 29 | 10% |
Student > Doctoral Student | 26 | 9% |
Student > Master | 26 | 9% |
Other | 28 | 10% |
Unknown | 66 | 23% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 55 | 19% |
Agricultural and Biological Sciences | 50 | 18% |
Pharmacology, Toxicology and Pharmaceutical Science | 30 | 11% |
Medicine and Dentistry | 20 | 7% |
Chemistry | 17 | 6% |
Other | 33 | 12% |
Unknown | 78 | 28% |