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Mendeley readers
| Chapter title |
Mitochondrial Membrane Potential Assay
|
|---|---|
| Chapter number | 2 |
| Book title |
High-Throughput Screening Assays in Toxicology
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-6346-1_2 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6344-7, 978-1-4939-6346-1
|
| Authors |
Srilatha Sakamuru, Matias S. Attene-Ramos, Menghang Xia, Sakamuru, Srilatha, Attene-Ramos, Matias S., Xia, Menghang |
| Abstract |
Mitochondrial function, a key indicator of cell health, can be assessed by monitoring changes in mitochondrial membrane potential (MMP). Cationic fluorescent dyes are commonly used tools to assess MMP. We used a water-soluble mitochondrial membrane potential indicator (m-MPI) to detect changes in MMP in HepG2 cells. A homogenous cell-based MMP assay was optimized and performed in a 1536-well plate format to screen several compound libraries for mitochondrial toxicity by evaluating the effects of chemical compounds on MMP. |
Mendeley readers
The data shown below were compiled from readership statistics for 278 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Portugal | 3 | 1% |
| Germany | 1 | <1% |
| South Africa | 1 | <1% |
| United Kingdom | 1 | <1% |
| Poland | 1 | <1% |
| Unknown | 271 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 74 | 27% |
| Researcher | 33 | 12% |
| Student > Bachelor | 29 | 10% |
| Student > Doctoral Student | 26 | 9% |
| Student > Master | 25 | 9% |
| Other | 29 | 10% |
| Unknown | 62 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 53 | 19% |
| Agricultural and Biological Sciences | 50 | 18% |
| Pharmacology, Toxicology and Pharmaceutical Science | 30 | 11% |
| Medicine and Dentistry | 20 | 7% |
| Chemistry | 17 | 6% |
| Other | 34 | 12% |
| Unknown | 74 | 27% |