Chapter title |
Two-Photon Imaging of T-Cell Motility in Lymph Nodes: In Vivo and Ex Vivo Approaches
|
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Chapter number | 5 |
Book title |
Intravital Imaging of Dynamic Bone and Immune Systems
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Published by |
Humana Press, New York, NY, February 2018
|
DOI | 10.1007/978-1-4939-7762-8_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7761-1, 978-1-4939-7762-8
|
Authors |
Akira Takeda, Masayuki Miyasaka, Eiji Umemoto |
Abstract |
T-cell motility is essential for the T cells' ability to scan antigens within lymph nodes and initiate contact with antigen-presenting cells. While T-cell migration has been extensively studied using in vitro migration assays, accumulating evidence indicates that the T-cell migration within lymph nodes is modulated by the surrounding cells and extracellular matrix, which form the confined architecture of the lymph nodes. Therefore, to understand the mechanisms of T-cell motility in vivo, their cell migration must be analyzed under physiological conditions. To this end, two-photon microscopy is extremely useful; this technique enables the tracking of fluorescently labeled cells in vivo and ex vivo, with high spatial and temporal resolutions. Here we describe the experimental procedures for applying two-photon microscopy to the in vivo and ex vivo imaging of T-cell migration in mouse lymph nodes. These approaches provide physiological insight into the mechanisms of T-cell behavior at a single-cell level in the three-dimensional lymph node environment. |
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Geographical breakdown
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Other | 1 | 17% |
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Unknown | 3 | 50% |
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