| Chapter title |
New Tools for Imaging of Immune Systems: Visualization of Cell Cycle, Cell Death, and Cell Movement by Using the Mice Lines Expressing Fucci, SCAT3.1, and Kaede and KikGR
|
|---|---|
| Chapter number | 16 |
| Book title |
Intravital Imaging of Dynamic Bone and Immune Systems
|
| Published by |
Humana Press, New York, NY, February 2018
|
| DOI | 10.1007/978-1-4939-7762-8_16 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7761-1, 978-1-4939-7762-8
|
| Authors |
Michio Tomura |
| Abstract |
Visualization of biological events in real time in vivo has become a crucial to understand immune responses. We have been established novel visualization tools for life of immune cells: proliferation, cell death, and migration. Fucci-transgenic mice allow us to visualize cell cycle phases by reciprocal expression of mKusabira-Orange2 in G1phase and mAzami-Green in S/G2/M phase. Caspase-3 indicator SCAT3.1 knock-in mice visualize cell death by changing color. Photoconvertible proteins, Kaede and KikGR expressing mice track cell movement between organs by labeling immune cells as red color. Here, I will introduce how to use and visualize these mice. These techniques will help to understand immune system in the living whole body. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 11 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 27% |
| Professor | 2 | 18% |
| Student > Bachelor | 2 | 18% |
| Student > Ph. D. Student | 2 | 18% |
| Professor > Associate Professor | 1 | 9% |
| Other | 0 | 0% |
| Unknown | 1 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 4 | 36% |
| Biochemistry, Genetics and Molecular Biology | 1 | 9% |
| Agricultural and Biological Sciences | 1 | 9% |
| Economics, Econometrics and Finance | 1 | 9% |
| Medicine and Dentistry | 1 | 9% |
| Other | 1 | 9% |
| Unknown | 2 | 18% |