| Chapter title |
Live Imaging of Interstitial T Cell Migration Using Lymph Node Slices
|
|---|---|
| Chapter number | 4 |
| Book title |
Intravital Imaging of Dynamic Bone and Immune Systems
|
| Published by |
Humana Press, New York, NY, February 2018
|
| DOI | 10.1007/978-1-4939-7762-8_4 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7761-1, 978-1-4939-7762-8
|
| Authors |
Tomoya Katakai |
| Abstract |
Live imaging using various microscopic technologies is an indispensable tool for investigating the dynamic nature of immune cells. One of the most powerful techniques is the two-photon laser-scanning microscopy (TP-LSM), which has various advantages in observing deep tissues in vivo. Interstitial T cell migration in the lymph node (LN) is a phenomenon intensively examined using TP-LSM in the field of immunology. Intravital and explant methods have been standards for imaging T cell behaviors in the LN, though there are several limitations. Live imaging of LN slices, an LN explant sliced by a vibratome to expose tissue parenchyma, could provide an alternative approach with technical advantages for an in-depth analysis of interstitial T cell migration in vivo. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 4 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 3 | 75% |
| Professor | 1 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 2 | 50% |
| Biochemistry, Genetics and Molecular Biology | 1 | 25% |
| Neuroscience | 1 | 25% |