Chapter title |
Intravital Imaging of Mouse Bone Marrow: Hemodynamics and Vascular Permeability
|
---|---|
Chapter number | 2 |
Book title |
Intravital Imaging of Dynamic Bone and Immune Systems
|
Published by |
Humana Press, New York, NY, February 2018
|
DOI | 10.1007/978-1-4939-7762-8_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7761-1, 978-1-4939-7762-8
|
Authors |
Yookyung Jung, Joel A. Spencer, Anthony P. Raphael, Juwell W. Wu, Clemens Alt, Judith R. Runnels, Briaira Geiger, Charles P. Lin |
Abstract |
The bone marrow is a unique microenvironment where blood cells are produced and released into the circulation. At the top of the blood cell lineage are the hematopoietic stem cells (HSC), which are thought to reside in close association with the bone marrow vascular endothelial cells (Morrison and Scadden, Nature 505:327-334, 2014). Recent efforts at characterizing the HSC niche have prompted us to make close examinations of two distinct types of blood vessel in the bone marrow, the arteriolar vessels originating from arteries and sinusoidal vessels connected to veins. We found the two vessel types to exhibit different vascular permeabilites, hemodynamics, cell trafficking behaviors, and oxygen content (Itkin et al., Nature 532:323-328, 2016; Spencer et al., Nature 508:269-273, 2014). Here, we describe a method to quantitatively measure the permeability and hemodynamics of arterioles and sinusoids in murine calvarial bone marrow using intravital microscopy. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 2 | 25% |
Student > Ph. D. Student | 2 | 25% |
Professor | 1 | 13% |
Student > Master | 1 | 13% |
Researcher | 1 | 13% |
Other | 0 | 0% |
Unknown | 1 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Engineering | 2 | 25% |
Agricultural and Biological Sciences | 1 | 13% |
Nursing and Health Professions | 1 | 13% |
Physics and Astronomy | 1 | 13% |
Immunology and Microbiology | 1 | 13% |
Other | 0 | 0% |
Unknown | 2 | 25% |